General phytochemical screening of the aerial parts of Leptadenia reticulata (Retz) Wight and Arn. (Asclepiadaceae) revealed the presence of steroids, terpenes, phenolic compounds, saponins, fatty acids, Flavonoids. The aim of this study is to identify and characterize the bioactive principle from the aerial parts of the plant. It has wide folk medicinal use. For isolation of the compound, the dried aerial parts powder of Leptadenia reticulata was subjected to hot extraction with petroleum ether this extract was saponified with alcoholic KOH and the unsaponifiable matter (LRUSM) from petroleum extract was subjected to column chromatography. Isolated compound were purified by chloroform. The isolation and purification afforded white crystalline powder which was subjected to physical, chemical and spectral identification by IR, 1H‐NMR, 13C‐NMR and GC‐MS. From the above physical, chemical and spectral evidences, the compounds isolated from unsaponifiable matter of the aerial parts Leptadenia Reticulata is confirmed as Campetstrol and β-sitosterol. They were isolated and chemical structures elucidated respectively. It was carried out by means of various physic chemical method, solvent extraction, HPTLC, Column chromatography and spectral hypanated techniques GC-MS study.
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